A rabbit monoclonal antibody-mediated lateral flow immunoassay for rapid detection of CTX-M extended-spectrum β-lactamase-producing Enterobacterales

Infections of CTX-M extended-spectrum β-lactamase-producing Enterobacterales are a severe threat in clinical settings. genes on plasmids have been transferred to many species, and these species spread, leading the global problem antimicrobial resistance. Here, we developed lateral flow immunoassay (LFIA) based an anti-CTX-M rabbit monoclonal antibody. This antibody detected variants from CTX-M-9, CTX-M-2, CTX-M-1 groups expressed isolates. The LFIA showed 100% sensitivity specificity with isolates agar plates, its limit detection was 0.8 ng/mL recombinant CTX-M-14. did not cross-react bacteria producing other class A β-lactamases, including SHV. In conclusion, highly sensitive specific capable detecting enzyme production Enterobacterales. We anticipate that our will become point-of-care test enabling rapid hospital community settings as well environmental test.